Urease and β-glucosidase activity enhanced the transformation of functional groups of humin amended by straw and straw-derived biochar

Abstract Background Organic materials carbon is mainly sequestrated in humin. However, the (C) stability of humin with organic amendments different types soils affected by or soil as well enzymatic reaction important for understanding C sequestration mechanism soil. Results In this study, straw and straw-derived biochar were incorporated into three soils. Using 13 nuclear magnetic resonance (NMR) spectroscopy, structural composition (Hu) was analyzed, along urease β-glucosidase activities. The results showed that amendment enhanced aryl to 49.63%–76.55% while increased O-alkyl Hu 33.99%–51.70%. Two-way ANOVA analysis revealed significant differences impact treatments on enzyme activities functional groups A positive correlation found between activity C, phenolic methoxyl addition, a alkyl Compared tobacco straw, straw-biochar 22.01 mg kg −1 d , 26.01 14.01 soils, respectively. Conclusions These contributed transformation influenced types, especially types. findings imply application stimulates activities, which improves alternation Graphical